Introduction: Oxidative stress has been suggested to play a role indemyelination and axonal damage in patients with MS. Vitamin D and swimming can reduce oxidative damages and help in the maintenance of brain cells. Klotho, protects neurons against the oxidative stress and demyelination.
Methodology: Female Lewis rats four weeks were divided into 9 groups:1- control sedentary; 2- EAE sedentary; 3- sham; 4- control vitamin D؛ 5- EAE vitamin D; 6- control exercise; 7- EAE +exercise; 8-Supplementation and healthy swim training and 9- EAE+ exercise+ vitamin D. Swim training began with 30 minutes those with higher daily five-minute workout time, the second week was 60 minutes and this time was fixed to the end of the training period. During the training period with an unchanged 60 Minutes, In order to overload training, Speed and power flow of 7 liters to 20 liters per minute increased. supplement Group received the amount of 2 ng of vitamin D for every other day. In order to induce EAE, Hindi guinea pig spinal cord were and 1: 1 with the adjuvant purchased from Sigma, were mixed and anesthetized the animals,4/0 Ml of the solution to each animal, and EAE model injected the base of the tail, and induced at the beginning of the seventh week of training. Immediately after the onset of symptoms, a biopsy was performed. Analyze data using ONE WAY ANOVA and Tukey POST HOC test
Results:there was difference significantly in Klotho levels of brain tissue of EAE sedentary compared with healthy control group (P=0.001). mean difference of EAE exercise group compared with the control group was not significant (P≥0.05). there was significant difference in Klotho levels of the brain tissue in EAE supplementation group compared with the control group (P=0.001).
Discussion: Pre-treatment with swimming training cant maintain the level of Klotho against EAE is induced neuronal damage But injections of vitamin D supplementation alone and in combination with exercise, increases the level of Klotho and protective effect on Klotho levels brain tissue and against neurotoxin EAE.