Introduction: Patellofemoral pain syndrome, cause disturbance in pain and performance. The aim of this study was to compare the effect of quadriceps muscles strength and stretch of gastrocnemius and soleus muscle on pain and functional disability in patients with Patellofemoral pain syndrome. For this purpose, 40 patients were females Patellofemoral pain syndrome who were referred to the center of Shariati Hospital, 20 individuals are chosen randomly and evenly in the first two groups (age: 2.1 ± 57.32 years, height: 7.2 ± 173 cm, weight 4.5 ± 68 kg) and the second experimental group (4.1 ± 43 years, height: 2.2 ± 164 cm, 6.2 ± 69 kg) respectively. The first experimental group for eight weeks, quadriceps muscle strengthening exercises and stretching exercises muscles in the same period of the second experimental group did the twins and soleus. Before and after the training period VAS and WOMAC pain and function were assessed by questionnaire. To analyze the data and independent t tests were used. Especially significant improvement in pain after exercise training group was observed (P<0.05). So that between the first experimental group before pain (X=8±0.6) and after (X=5.46±1/2) there was a significant difference (P<0.05). Pain between the two groups before (X=7.57±1.2) and after (X=5.14±1.4) there was a significant difference ((P<0.05). In the case of performance between the performance of the experimental group before X=37.85±0.9) and after (X=32.57±1.2) there was a significant difference (05.0> P). Between the performance of the experimental group before (X=32.28±2.04) and after (X=22.28±1.02) there was a significant difference (P<0.05). Sports rehabilitation specialists recommend Patellofemoral pain syndrome in patients with muscle strengthening exercises in addition to quadriceps strengthening, twins and soleus muscle stretching exercises to use.