Introduction: Nowadays, breast cancer is an important risk factor for women’s health. Regular exercise has been demonstrated to considerably lead to inhibition of tumor growth and mitigation of metastatic expansion in animals and humans. Let-7 has also been shown to be significantly correlated with the occurrence and development of breast cancer and that it is involved in the regulation of oncogenic pathways in numerous types of tumors. Let-7 is, therefore, a potential molecular target for tumor therapy. HMGA as a target oncogene of let-7. Previous study demonstrate that let-7a negatively mediates the expres‌sion of the oncogene HMGA2 by binding to its 3' UTR and directly decreasing its expression. Therefore ,the aim of this study was the investigation of the effect of 10 weeks of high intensity interval training (HIT) on the expression Let-7a and HMGA2 in tumor tissue in breast cancer bearing mice.
Methodology: 12 adult female mice whit the tumor cell lie MC4-L2 divided to three groups control, and high intensity interval training for 10 weeks. Tumor volume was calculated by sliding caliper using the formula joined. In addition, gene expression of Let-7a and HMGA2 were assessment by Real time-PCR and the quantification of gene expression levels using The Differences between groups were analyzed by independent T –test.
Result: The statistical results showed that HIT subsequently increased the expression of Let-7a and significantly reduced the HMGA2 gene expression in the training group compared to the control group(p<0.05)
Discussion: The results of this study indicated that 10 weeks of high intensity interval training (HIT) by increasing the expression of Let-7a and reducing the HMGA2 gene expression causes decrease of tumor growth rate in breast cancer bearing mice