Introduction: The mitochondrial uncoupling proteins (UCPs) of brown adipose tissue (BAT) play an important role in energy balance. In this manner UCP1 provides the ability for BAT to release excess energy from fat depots and glucose as heat. UCP1 mRNA is a contain of mitochondria which mainly express in BAT. This mRNA disappears the electrochemical gradient in electron transport chain and uncouple the respiration with releasing heat. Recent studies demonstrated that physical activity caused an increase in PGC-1α and FNDC5 which was followed by an increase in UCP1 content and thermogenesis. Also elevated stimulation of SNS during exercise was observed and demonstrated that BAT was highly active in exercise training. Therefore, the aim of present study was to determine the effect of endurance training on the expression of UCP1 mRNA in brown adipose tissue of obese male C57BL/6 mice.
Methodology: 12 male mice were fed high fat diet during 12 weeks. They divided into two same (HF/HF-EX)(HF/HF- SED) randomly. 24 hours after the last practice session they sacrificed and their BAT isolated for gene expression with real time PCR.
Results: result showed a significant increase in UCP1 level in BAT of trained group due to 8 week endurance training in contrast SED group.
Discussion: Recently it has been shown that UCP1 expression due to HFD consumption increases, which in this study it was more obvious in EX group. Increased level of UCP1 in BAT which is the result of endurance training in obese mice probably can illustrate the benefits of training on weight loss and metabolic disorders symptoms.