Introduction: It seems that Heat shock protein-70 (HSP70) as a cellular and molecular helper plays a considerable role in the biochemical and physiological processes of cardiac hypertrophy and growth of cardiac myocytes. The effects of exercise on skeletal muscle increased HSP70 and play an important role in adaptation to stress. During the research on professional trained rowers, Liu et al reported that HSP70 was induced by high-intensity training, but not by endurance training at low intensity. Thus, the purpose of this study was to investigate the effect of 8 weeks HIIT aerobic training on HSP70 gene expression as a messenger stimulus of cardiac cells in sedentary young men.
Methodology: This investigation is a semi-experimental study. Statistical population was all healthy and sedentary students 21-30 years old of Cultural University and Technical College in Urmia city. Twenty-one subjects with an average 25 years and weight 83 kg voluntarily participated in this study, and then subjects were randomly divided into two the Exercise (9 men) and Control (11 men) groups. Exercise group performed of 8 weeks and three sessions per week high-intensity interval training (HIIT) aerobic exercises 60-90% maximal heart rate of reserve on a treadmill with zero slopes, but the control group participated in no intervention. Twenty-four before and after the 8 weeks training program evaluated to measure HSP70 gene expression by real-time RT-PCR machine were taken blood samples in the Exercise & Control Groups. Data analysis included descriptive and inferential (independent T-test) statistics using SPSS-23 software and a significance level of P≥0.05 was considered.
Results: The results showed that HSP70 gene expression after 8 weeks of HIIT aerobic exercises in the exercise group compared with the control group had no significant difference(P>0.05). Moreover, in the inter-group results was no significant difference (P>0.05). Discussion: Results showed that 8 weeks of HIIT aerobic exercise had no significant effect on HSP70 gene expression in sedentary young men that consistent and inconsistent with Shastry et al (2002) and Liu et al (2004) results, respectively. The causes of consistent and inconsistent can be age and sex of subjects and a different type of subject, respectively. The results suggested that 8 weeks of HIIT aerobic exercises %60-90 maximum heart rate of reserve on HSP70 gene expression did not change, and thus the need for further researches in this field.